Alexandre GUIRONNET

PhD student

Contact

alexandre.guironnet[@]isa-lyon.fr

Research

Alexandre Guironnet is currently a PhD student under the supervision of Dr.Emmanuelle Vulliet and Laure Wiest, research engineer. His research is focused on the development of chromatographic methods for the detection and quantification of veterinary antibiotics and biocides in solid environmental matrices such as sewage sludge, using LC-MS/MS. Three different antibiotic classes will be considered: aminoglycosides, penicillin and polymixins.These classes were selected due to their challenging analytical nature, regarding both analysis and sample preparation. Furthermore, sewage sludge is a very complex matrix, thus extraction procedures will be thoroughly experimented.

Biography

Alexandre Guironnet graduated in 2018 from both the École Supérieure de Chimie Physique Électronique (CPE Lyon engineer diploma (MSc) in chemistry – process engineering) and the Université Claude Bernard Lyon 1 (Master degree in analytical chemistry).
He worked in 2016-2017 during one year in the United Kingdom for Syngenta, an agrochemical company, in their Research and Development Centre and did his 6 months master final project from March to September 2018 in the Isotopic and Organic platform at ISA before starting his doctoral studies under the supervision of Emmanuelle Vulliet in the “Technology and Research in Analytical Chemistry for Environment, health and their interactions” team

Keywords

Antibiotics, environment, sample prep, LC-MS/MS

Publications

Publications HAL de Alexandre,Guironnet

2022

Journal articles

ref_biblio
Concepcion Sanchez-Cid, Alexandre Guironnet, Christoph Keuschnig, Laure Wiest, Emmanuelle Vulliet, et al.. Gentamicin at sub-inhibitory concentrations selects for antibiotic resistance in the environment. ISME Communications, 2022, 2 (1), pp.29. ⟨10.1038/s43705-022-00101-y⟩. ⟨hal-03746450⟩
Accès au texte intégral et bibtex
https://hal.science/hal-03746450/file/ISME%20Communications.pdf BibTex
ref_biblio
Alexandre Guironnet, Laure Wiest, Emmanuelle Vulliet. Advantages of MS/MS/MS (MRM3) vs classic MRM quantification for complex environmental matrices: Analysis of beta-lactams in WWTP sludge. Analytica Chimica Acta, 2022, 1205, pp.339773. ⟨10.1016/j.aca.2022.339773⟩. ⟨hal-03745350⟩
Accès au texte intégral et bibtex
https://hal.science/hal-03745350/file/Guironnet%282022%29-ACA.pdf BibTex
ref_biblio
Alexandre Guironnet, Laure Wiest, Emmanuelle Vulliet. Improvement of the QuEChERS extraction step by matrix-dispersion effect and application on beta-lactams analysis in wastewater sludge by LC-MS/MS. Talanta, 2022, 237, pp.122923. ⟨10.1016/j.talanta.2021.122923⟩. ⟨hal-03508563⟩
Accès au texte intégral et bibtex
https://hal.science/hal-03508563/file/Fichier%20auteur.pdf BibTex

2021

Journal articles

ref_biblio
Alexandre Guironnet, Concepcion Sanchez-Cid, Timothy M. Vogel, Laure Wiest, Emmanuelle Vulliet. Aminoglycosides analysis optimization using Ion pairing Liquid Chromatography coupled to tandem Mass Spectrometry and application on wastewater samples. Journal of Chromatography A, 2021, pp.462133. ⟨10.1016/j.chroma.2021.462133⟩. ⟨hal-03200052⟩
Accès au texte intégral et bibtex
https://hal.science/hal-03200052/file/2021_JChromatogrA_Aminoglycosides-analysis-optimization_E-Vulliet.pdf BibTex
ref_biblio
Concepcion Sanchez-Cid, Alexandre Guironnet, Laure Wiest, Emmanuelle Vulliet, Timothy M. Vogel. Gentamicin Adsorption onto Soil Particles Prevents Overall Short-Term Effects on the Soil Microbiome and Resistome. Antibiotics, 2021, Special Issue The Distribution of Antibiotic Resistance in Terrestrial Ecosystems, 10 (2), pp.191. ⟨10.3390/antibiotics10020191⟩. ⟨hal-03200197⟩
Accès au texte intégral et bibtex
https://hal.science/hal-03200197/file/Sanchez-Cid%202021%20Antibiotics.pdf BibTex

2020

Journal articles

ref_biblio
François Fourel, Christophe Lécuyer, Patrick Jame, Alexandre Guironnet, Antoine Boutier, et al.. Simultaneous δ2H and δ18O analyses of water inclusions in halite with Off-Axis Integrated Cavity Output Spectroscopy (OA-ICOS). Journal of Mass Spectrometry, 2020, 55, pp.e4615. ⟨10.1002/jms.4615⟩. ⟨hal-02891946⟩
Accès au bibtex
BibTex