Yoan R. MONNEAU
I am most attracted to critical testing of proposed molecular mechanisms to explain biological functions. To address this issue, I aim to characterize at atomic scale structures and dynamics of biologically relevant protein systems by NMR spectroscopy and other biophysical means. In order to get insight on challenging systems, I have a keen interest in developing new methods to expand the scope of liquid state NMR.
Main topics and collaborations:
Chemical characterization of hyaluronic hydrogel (ALLERGAN, Annecy)
Methyl-aided liquid state NMR of complex protein systems (BIOSYS, ISA, Lyon)
Measured and simulated molecular dynamics of methyls (BIOSYS, ISA, Lyon)
Methyl-labeling schemes and algorithms to expand the scope of liquid state NMR
(Paolo Rossi and Babis Kalodimos at the NMR lab of St Jude Children Research Hospital, Memphis TN, USA)
Interaction between glycosaminoglycans and proteins (SAGAG, IBS, Grenoble)
Yoan R. Monneau education has focused on learning biophysics methods to reveal molecular mechanisms that underlie observable phenotypes. He was graduated summa cum laude in biochemistry, and more specifically, in structural biology and biomolecular nuclear magnetic resonance (NMR), from Bordeaux University in December 2011, under the supervision of Dr. Cameron Mackereth, group leader at the European Institute of chemistry-Biology (IECB, Pessac, France). His PhD project was about to understand impacts of post-translational modifications on histone structures and dynamics. This PhD program extensively trained him in NMR and recombinant protein production/purification.
After his PhD, motivated by the desire to improve his skills and knowledge in biomolecular NMR, especially to study large molecular protein systems using liquid state NMR, Yoan joined the research group of Charalampos (Babis) Kalodimos, a worldwide leading laboratory in this field, at Rutgers University (Piscataway NJ, USA) in September 2012. The research program was about to decipher how the 200kDa nano-machine SecA can convert chemical energy to mechanical energy and how such protein can handle hundreds of different client proteins and translocate them through the bacterial membrane. Liquid state NMR on such big system relies on protein methyl labeling in a perdeuterated background. Considering that methyl assignment is the major bottleneck of such project, he has independently initiated two side research projects: the development of an alternative methyl-labeling scheme for either Leucine or Valine using newly-designed auxotroph E. coli strains; and an automatic assignment procedure based on the matching between a peak-peak correlation network, defined according to NOESY data, and a methyl-methyl vicinity network, defined according to protein structure.
On April 2014, He then joined, as a scholar in biomolecular NMR, the laboratory of Dr. Hugues Lortat-Jacob at the Institute of Structural Biology (UMR 5075, IBS, Grenoble, France). He was initially hired to work on the chemokine CXCL13 in order to define the role of heparan sulfates (HS) in its function. However, his work expands to the investigation of HS structure-function relationships by specifically addressing how HS, by its interactions with a variety of proteins (cytokines, Growth factors, chemokines, etc.) can regulate their functions and how HS biosynthesis processes regulate HS modification patterns needed to interact with all those above-mentioned proteins.
Finally, He joined on April 2019 as post-doctoral follow, the team of Dr. Olivier Walker, the “BIOphysics of complex SYStems” group (BIOSYS), at the Institute of Analytical Sciences (UMR 5280, CNRS, ENS, University Claude Bernard Lyon I).
Articles in peer-review journals (as researcher at ISA UMR 5280)
Articles in peer-review journals (non-afiliated to ISA)
Substrate binding mode and catalytic mechanism of human heparan sulfate d-glucuronyl C5 epimerase
Claire Debarnot, Yoan R Monneau, Véronique Roig-Zamboni, Vincent Delauzun, Christine Le Narvor, Emeline Richard, Jérôme Hénault, Adeline Goulet, Firas Fadel, Romain R Vivès, Bernard Priem, David Bonnaffé, Hugues Lortat-Jacob, Yves Bourne
Proceedings of the National Academy of Sciences, 2019, 116 (14), 6760-6765
Toolkit for NMR Studies of Methyl-Labeled Proteins.
Paolo Rossi, Yoan R Monneau, Youlin Xia, Yojiro Ishida, Charalampos G Kalodimos
Methods in enzymology, 2019, 614, 107-142
Automatic methyl assignment in large proteins by the MAGIC algorithm
Yoan R Monneau, Paolo Rossi, Anusarka Bhaumik, Chengdong Huang, Yajun Jiang, Tamjeed Saleh, Tao Xie, Qiong Xing, Charalampos G Kalodimos
Journal of biomolecular NMR, 2017, 69 (4), 215-227
Basic surface features of nuclear FKBPs facilitate chromatin binding
Andrew Leung, Francy-Pesek Jardim, Neda Savic, Yoan R Monneau, Rodrigo González-Romero, Geoff Gudavicius, Jose M Eirin-Lopez, Till Bartke, Cameron D Mackereth, Juan Ausió, Christopher J Nelson
Scientific reports, 2017, 7 (1), 3795
Epithelial chemokine CXCL14 synergizes with CXCL12 via allosteric modulation of CXCR4
Paul J Collins, Michelle L McCully, Laura Martínez-Muñoz, César Santiago, James Wheeldon, Stephan Caucheteux, Sylvia Thelen, Valentina Cecchinato, Julia M Laufer, Vladimir Purvanov, Yoan R Monneau, Hugues Lortat-Jacob, Daniel F Legler, Mariagrazia Uguccioni, Marcus Thelen, Vincent Piguet, Mario Mellado, Bernhard Moser
The FASEB Journal, 2017, 31 (7), 3084-3097
Solution structure of CXCL13 and heparan sulfate binding show that GAG binding site and cellular signalling rely on distinct domains
Yoan R Monneau, Lingjie Luo, Nehru Viji Sankaranarayanan, Balaji Nagarajan, Romain R Vivès, Françoise Baleux, Umesh R Desai, Fernando Arenzana-Seidedos, Hugues Lortat-Jacob
Open biology, 2017, 7 (10), 170133
Exploiting E. coli auxotrophs for leucine, valine, and threonine specific methyl labeling of large proteins for NMR applications
Yoan R Monneau, Yojiro Ishida, Paolo Rossi, Tomohide Saio, Shiou-Ru Tzeng, Masayori Inouye, Charalampos G Kalodimos
Journal of biomolecular NMR, 2016, 65 (2), 99-108
The sweet spot: how GAGs help chemokines guide migrating cells
Yoan R Monneau, Fernando Arenzana‐Seisdedos, Hugues Lortat‐Jacob
Journal of leukocyte biology, 2016, 99 (6), 935-953
Structure and activity of the peptidyl-prolyl isomerase domain from the histone chaperone Fpr4 toward histone H3 proline isomerization
Yoan R Monneau, Heddy Soufari, Christopher J Nelson, Cameron D Mackereth
Journal of Biological Chemistry, 2013, 288 (36), 25826-25837
Chemical shift assignments of the catalytic domain from the yeast proline isomerase Fpr4p
Yoan R Monneau, Christopher J Nelson, Cameron D Mackereth
Biomolecular NMR assignments, 2012, 6 (2), 123-126