Associate Professor




Methods development for the characterization of virulence factors in bacteria by targeted proteomics.

Applications of MS-based lipidomics techniques (global lipidome, targeted approach)

Grants :

  • ANR JCJC (2019 – 2021) – coordinator : Proteomics and Lipidomics Approaches for understanding molecular mechanisms of enviroNmental chemical TOXicity in conjunction with lipid metabolism in G. fossarum during reproduction cycle (PLAN-TOX)
  • ANR PRCE (2018 – 2022) – partner : Démarche intégrée pour proposer la Protéomique dans la surveillance : accumulation, devenir et multimarqueurs (APPROve)

Collaborations : Irstea (Ecotoxicology group), ICBMS (MEM² group), IBCP (Suzana Salcedo group), MPI-CBG (Shevchenko group), ICSN (David Touboul group), IPN (Serge Della-Negra)



Sophie Ayciriex earned her M.Sc. degrees in Biochemistry and Structural Biology – Structure/Function in Macromolecules and Living Processes in 2006 and 2007 respectively, at the Institute of Pharmacology and Structural Biology (IPBS/CNRS, UMR 5089) and University Paul Sabatier (Toulouse, France).

She obtained her European Ph.D. degree in Biochemistry in 2010 at the University of Bordeaux. During her PhD fellowship (2007-2010), she investigated the characterization of new acyltransferases in yeast by reverse genetics and Lipidomics analyses (mass spectrometry). With an EMBO short-term fellowship (ASTF number 373–2008), she explored the advantages of shotgun-lipidomics on high-resolution mass spectrometry through a collaborative project with the Mass Spectrometry Laboratory of Andrej Shevchenko (Max Planck Institute of Molecular Cell Biology and Genetics, MPI-CBG, Dresden, Germany).

For her first post-doctoral fellowship (2011-2012), she joined the laboratory of Organic, Medicinal and Extractive Chemistry and Experimental Toxicology – COMETE (UMR 8638 CNRS) at the Faculty of Pharmacy, University Paris Descartes in Paris, France. She explored the mapping of lipidome variations during neurodegenerative processes (collaborations: Prof. Charles Duyckaerts and Dr. Nathalie Cartier-Lacave).

Then, she went back to the laboratory of Andrej Shevchenko (MPI-CBG, Dresden) to investigate the lipid-protein interactions and the impact of diet on Drosophila photoreceptors during a second post-doctorate (2012-2015).

She joined in 2015 as associate professor, the team Biological Analysis by Mass Spectrometry headed by Prof. Jérôme Lemoine at the Institute of Analytical Sciences (UMR 5280, CNRS, University Claude Bernard Lyon I). Her main research interest deals with the quantitative analyses of biomolecules (proteins and lipids) by targeted mass spectrometry and high-resolution mass spectrometry.

  • Advisory Board SFSM ( : treasurer (2017-2020)
  • Editorial Board : Journal of Applied Bioanalysis (
  • Member of the scientific animation of the Institute of Analytical Sciences (2017-present) :
  • Local organizer of the French Lipidomics Network hosted at ISA (25-26th October 2018) : 8ème édition des Journées du Réseau des LipidomYstes –


Mass Spectrometry; Lipidomics; Targeted Proteomics; Multiplexed analysis; Untargeted approach; Ecotoxicology; Health (Alzheimer Disease, Polyarthritis…)


Publications HAL de Sophie,Ayciriex


Journal articles

Tingting Fu, Oskar Knittelfelder, Geffard Olivier, Yohann Clément, Eric Testet, et al.. Shotgun lipidomics and mass spectrometry imaging unveil diversity and dynamics in Gammarus fossarum lipid composition. iScience, Elsevier, 2021, 24 (2), pp.102115. ⟨10.1016/j.isci.2021.102115⟩. ⟨hal-03134038⟩
Accès au bibtex


Journal articles

Ivano Di Meo, Chiara Cavestro, Silvia Pedretti, Tingting Fu, Simona Ligorio, et al.. Neuronal Ablation of CoA Synthase Causes Motor Deficits, Iron Dyshomeostasis, and Mitochondrial Dysfunctions in a CoPAN Mouse Model. International Journal of Molecular Sciences, MDPI, 2020, 21 (24), pp.9707. ⟨10.3390/ijms21249707⟩. ⟨hal-03116877⟩
Accès au texte intégral et bibtex BibTex
Tingting Fu, Janina Oetjen, Manuel Chapelle, Alexandre Verdu, Matthias Szesny, et al.. In situ isobaric lipid mapping by MALDI-ion mobility separation-mass spectrometry imaging. Journal of Mass Spectrometry, Wiley-Blackwell, 2020, 55 (9), pp.e4531. ⟨10.1002/jms.4531⟩. ⟨hal-03113280⟩
Accès au bibtex
Julien Faugere, Duarte Gouveia, Sophie Ayciriex, Arnaud Chaumot, Christine Almunia, et al.. High-multiplexed monitoring of protein biomarkers in the sentinel Gammarus fossarum by targeted scout-MRM assay, a new vision for ecotoxicoproteomics. Journal of Proteomics, Elsevier, 2020, 226, ⟨10.1016/j.jprot.2020.103901⟩. ⟨hal-02903090⟩
Accès au bibtex
Arnaud Salvador, Romain Carrière, Sophie Ayciriex, Christelle Margoum, Yves Leblanc, et al.. Scout-multiple reaction monitoring: A liquid chromatography tandem mass spectrometry approach for multi-residue pesticide analysis without time scheduling. Journal of Chromatography A, Elsevier, 2020, 1621, pp.461046. ⟨10.1016/j.chroma.2020.461046⟩. ⟨hal-02771252⟩
Accès au bibtex
Sophie Ayciriex, Romain Carrière, Chloé Bardet, Yves Le Blanc, Arnaud Salvador, et al.. Streamlined Development of Targeted Mass Spectrometry‐Based Method Combining Scout‐MRM and a Web‐Based Tool Indexed with Scout Peptides. Proteomics, Wiley-VCH Verlag, 2020, pp.1900254. ⟨10.1002/pmic.201900254⟩. ⟨hal-02436928⟩
Accès au bibtex


Journal articles

Aurore Charnot, Duarte Gouveia, Sophie Ayciriex, Jérôme Lemoine, J. Armengaud, et al.. On-Line Solid Phase Extraction Liquid Chromatography-Mass Spectrometry Method for Multiplexed Proteins Quantitation in an Ecotoxicology Test Specie: Gammarus fossarum. Journal of Applied Bioanalysis, Betasciencepress Publishers, 2018, 4 (3), pp.81-101. ⟨10.17145/jab.18.012⟩. ⟨hal-01860702⟩
Accès au bibtex


Sophie Ayciriex, Fathia Djelti, Sandro Alves, Anne Regazzetti, Mathieu Gaudin, Jennifer Varin, Dominique Langui, Ivan Bièche, Eloise Hudry, Delphine Dargere, Patrick Aubourg, Nicolas Auzeil, Olivier Laprévote, Nathalie Cartier. Neuronal cholesterol accumulation induced by Cyp46a1 down-regulation in mouse hippocampus disrupts brain lipid homeostasis. Front. Mol. Neurosci. 2017 10:211. doi: 10.3389/fnmol.2017.00211

Blandine Rougemont, Sébastien Bontemps Gallo, Sophie Ayciriex, Romain Carrière, Hubert Hondermarck, et al. Scout-MRM: Multiplexed Targeted Mass Spectrometry-Based Assay without Retention Time Scheduling Exemplified by Dickeya dadantii Proteomic Analysis during Plant Infection. Anal Chem. 2017 Feb 7;89(3):1421-1426. doi: 10.1021/acs.analchem.6b03201.


Sophie Ayciriex, Hermeto Gerber, Guillermo M Garcia Osuna, Mohamed Chami, Henning Stahlberg, et al. The lipidome associated with the γ-secretase complex is required for its integrity and activity. Biochem J. 2016 Feb 1;473(3):321-34. doi: 10.1042/BJ20150448.


Marie-Anne Burlot, Jérôme Braudeau, Kristin Michaelsen-Preusse, Brigitte Potier, Sophie Ayciriex, et al. Cholesterol 24-hydroxylase defect is implicated in memory impairments associated with Alzheimer-like Tau pathology. Hum Mol Genet. 2015 Nov 1;24(21):5965-76. doi: 10.1093/hmg/ddv268.

Fathia Djelti, Jérôme Braudeau, Eloïse Hudry, Marc Dhenain, Jenifer Varin, et al. CYP46A1 inhibition, brain cholesterol accumulation and neurodegeneration pave the way for Alzheimer’s disease. Brain. 2015 Aug;138(Pt 8):2383-98. doi: 10.1093/brain/awv166.

Michael Habeck, Haim Haviv, Adriana Katz, Einat Kapri-Pardes, Sophie Ayciriex, et al. Stimulation, inhibition, or stabilization of Na,K-ATPase caused by specific lipid interactions at distinct sites. J Biol Chem. 2015 Feb 20;290(8):4829-42. doi: 10.1074/jbc.M114.611384. Epub 2014 Dec 22.


Jonathan Rodenfels, Oksana Lavrynenko*, Sophie Ayciriex*, Julio Sampaio*, Maria Carvalho, et al. Production of systemically circulating Hedgehog by the intestine couples nutrition to growth and development. Genes Dev. 2014 Dec 1;28(23):2636-51. doi: 10.1101/gad.249763.114.

Mathieu Gaudin, Maï Panchal, Sophie Ayciriex, Erwan Werner, Alain Brunelle, et al. Ultra-performance liquid chromatography – mass spectrometry studies of formalin-induced alterations of human brain lipidome. J Mass Spectrom. 2014 Oct;49(10):1035-42. doi: 10.1002/jms.3424.

Maï Panchal, Mathieu Gaudin, Adina N Lazar, Elisa Salvati, Isabelle Rivals, et al. Ceramides and sphingomyelinases in senile plaques. Neurobiol Dis. 2014 May;65:193-201. doi: 10.1016/j.nbd.2014.01.010.


Corinne Buré, Sophie Ayciriex, Eric Testet, Jean-Marie Schmitter. A single run LC-MS/MS method for phospholipidomics. Anal Bioanal Chem. 2013 Jan;405(1):203-13. doi: 10.1007/s00216-012-6466-9.


Sophie Ayciriex, Anne Regazzetti, Mathieu Gaudin, Elise Prost, Delphine Dargère, et al. Development of a novel method for quantification of sterols and oxysterols by UPLC-ESI-HRMS: application to a neuroinflammation rat model. Anal Bioanal Chem. 2012 Dec;404(10):3049-59. doi: 10.1007/s00216-012-6396-6.

Sophie Ayciriex, Marina Le Guédard, Nadine Camougrand, Gisèle Velours, Mario Schoene, et al. YPR139c/LOA1 encodes a novel lysophosphatidic acid acyltransferase associated with lipid droplets and involved in TAG homeostasis. Mol Biol Cell. 2012 Jan;23(2):233-46. doi: 10.1091/mbc.E11-07-0650.


Sophie Ayciriex, David Touboul, Alain Brunelle, Olivier Laprévote. Time-of-flight secondary ion mass spectrometer: a novel tool for lipid imaging. Clinical Lipidology 2011, 6 (4), 437-445


Marina Le Guédard, Jean-Jacques Bessoule, Valérie Boyer, Sophie Ayciriex, Gisèle Velours, et al. PSI1 is responsible for the stearic acid enrichment that is characteristic of phosphatidylinositol in yeast. FEBS J. 2009 Nov;276(21):6412-24. doi: 10.1111/j.1742-4658.2009.07355.x.