M. C. BONACCORSI, researcher from Sapienza Università di Roma will give a lecture on The yeast iron-responsive GATA factor Fep1: an intriguing [2Fe-2S] cluster protein the 27th june 2022 at the Institute for analytical science (11:00 am – seminar room).
ID meeting: 2733 086 9892 – Wordpass: 7pSFZpNH8d2
Iron-responsive transcription factors cooperate to maintain adequate levels of intracellular iron in order to make cells able to respond either to iron starvation or to excess. In a large number of fungi, transcriptional repressors sensitive to iron have been identified, which bind to a 5’-(A/T)GATA(A/G)-3’ sequence on DNA and are composed of two functionally distinct domains: an N-terminal DNA-binding domain and a C-terminal repression domain. Two zinc fingers of the Cys2-Cys2 type are present in the N-terminal DNA-binding domain, separated by a sequence that invariably contains a set of four cysteines within a Cys-X5-Cys-X8-Cys-X2-Cys motif that is strictly required for fungal GATA factors to be able to respond to iron levels.
Fep1, the iron-responsive GATA factor from the methylotrophic yeast Pichia pastoris, will be the subject of this seminar. Starting from the identification and characterization of the protein and the unusual properties of its [2Fe-2S] cluster cofactor, to a biochemical analysis of the complex of Fep1 with DNA, an overview of this intriguing protein will be provided