Sophie AYCIRIEX

Doctor
work phone: 0437423549

Research

Development of novel procedure by mass spectrometry for multiplex assay of proteins and lipids in the context of infectiology and ecotoxicology.

Biography

Sophie Ayciriex earned her M.Sc. degrees in Biochemistry and Structural Biology – Structure/Function in Macromolecules and Living Processes in 2006 and 2007 respectively, at the Institute of Pharmacology and Structural Biology (IPBS/CNRS, UMR 5089) and University Paul Sabatier (Toulouse, France).

She obtained her European Ph.D. degree in Biochemistry in 2010 at the University of Bordeaux. During her PhD fellowship (2007-2010), she investigated the characterization of new acyltransferases in yeast by reverse genetics and Lipidomics analyses (mass spectrometry). With an EMBO short-term fellowship (ASTF number 373–2008), she explored the advantages of shotgun-lipidomics on high-resolution mass spectrometry through a collaborative project with the Mass Spectrometry Laboratory of Andrej Shevchenko (Max Planck Institute of Molecular Cell Biology and Genetics, MPI-CBG, Dresden, Germany).

For her first post-doctoral fellowship (2011-2012), she joined the laboratory of Organic, Medicinal and Extractive Chemistry and Experimental Toxicology – COMETE (UMR 8638 CNRS) at the Faculty of Pharmacy, University Paris Descartes in Paris, France. She explored the mapping of lipidome variations during neurodegenerative processes (collaborations: Prof. Charles Duyckaerts and Dr. Nathalie Cartier-Lacave).

Then, she went back to the laboratory of Andrej Shevchenko (MPI-CBG, Dresden) to investigate the lipid-protein interactions and the impact of diet on Drosophila photoreceptors during a second post-doctorate (2012-2015).

She joined in 2015 as associate professor, the team Biological Analysis by Mass Spectrometry headed by Prof. Jérôme Lemoine at the Institute of Analytical Sciences (UMR 5280, CNRS, ENS, University Claude Bernard Lyon I). Her main research interest deals with the quantitative analyses of biomolecules (proteins and lipids) by targeted mass spectrometry and high-resolution mass spectrometry.

  • Advisory Board SFSM (http://www.sfsm.fr/) : treasurer (2017-2020)
  • Editorial Board : Journal of Applied Bioanalysis (https://jab.scholasticahq.com/)
  • Member of the scientific animation of the Institute of Analytical Sciences (2017-present) : umr5280.liste.coordination.animation@isa-lyon.fr

Keywords

Mass Spectrometry; Lipidomics; Targeted Proteomics; Multiplexed analysis; Untargeted approach; Ecotoxicology; Health (Alzheimer Disease, Polyarthritis…)

Publications

2017

Sophie Ayciriex, Fathia Djelti, Sandro Alves, Anne Regazzetti, Mathieu Gaudin, Jennifer Varin, Dominique Langui, Ivan Bièche, Eloise Hudry, Delphine Dargere, Patrick Aubourg, Nicolas Auzeil, Olivier Laprévote, Nathalie Cartier. Neuronal cholesterol accumulation induced by Cyp46a1 down-regulation in mouse hippocampus disrupts brain lipid homeostasis. Front. Mol. Neurosci. 2017 10:211. doi: 10.3389/fnmol.2017.00211

Blandine Rougemont, Sébastien Bontemps Gallo, Sophie Ayciriex, Romain Carrière, Hubert Hondermarck, et al. Scout-MRM: Multiplexed Targeted Mass Spectrometry-Based Assay without Retention Time Scheduling Exemplified by Dickeya dadantii Proteomic Analysis during Plant Infection. Anal Chem. 2017 Feb 7;89(3):1421-1426. doi: 10.1021/acs.analchem.6b03201.

2016

Sophie Ayciriex, Hermeto Gerber, Guillermo M Garcia Osuna, Mohamed Chami, Henning Stahlberg, et al. The lipidome associated with the γ-secretase complex is required for its integrity and activity. Biochem J. 2016 Feb 1;473(3):321-34. doi: 10.1042/BJ20150448.

2015

Marie-Anne Burlot, Jérôme Braudeau, Kristin Michaelsen-Preusse, Brigitte Potier, Sophie Ayciriex, et al. Cholesterol 24-hydroxylase defect is implicated in memory impairments associated with Alzheimer-like Tau pathology. Hum Mol Genet. 2015 Nov 1;24(21):5965-76. doi: 10.1093/hmg/ddv268.

Fathia Djelti, Jérôme Braudeau, Eloïse Hudry, Marc Dhenain, Jenifer Varin, et al. CYP46A1 inhibition, brain cholesterol accumulation and neurodegeneration pave the way for Alzheimer’s disease. Brain. 2015 Aug;138(Pt 8):2383-98. doi: 10.1093/brain/awv166.

Michael Habeck, Haim Haviv, Adriana Katz, Einat Kapri-Pardes, Sophie Ayciriex, et al. Stimulation, inhibition, or stabilization of Na,K-ATPase caused by specific lipid interactions at distinct sites. J Biol Chem. 2015 Feb 20;290(8):4829-42. doi: 10.1074/jbc.M114.611384. Epub 2014 Dec 22.

2014

Jonathan Rodenfels, Oksana Lavrynenko*, Sophie Ayciriex*, Julio Sampaio*, Maria Carvalho, et al. Production of systemically circulating Hedgehog by the intestine couples nutrition to growth and development. Genes Dev. 2014 Dec 1;28(23):2636-51. doi: 10.1101/gad.249763.114.

Mathieu Gaudin, Maï Panchal, Sophie Ayciriex, Erwan Werner, Alain Brunelle, et al. Ultra-performance liquid chromatography – mass spectrometry studies of formalin-induced alterations of human brain lipidome. J Mass Spectrom. 2014 Oct;49(10):1035-42. doi: 10.1002/jms.3424.

Maï Panchal, Mathieu Gaudin, Adina N Lazar, Elisa Salvati, Isabelle Rivals, et al. Ceramides and sphingomyelinases in senile plaques. Neurobiol Dis. 2014 May;65:193-201. doi: 10.1016/j.nbd.2014.01.010.

2013

Corinne Buré, Sophie Ayciriex, Eric Testet, Jean-Marie Schmitter. A single run LC-MS/MS method for phospholipidomics. Anal Bioanal Chem. 2013 Jan;405(1):203-13. doi: 10.1007/s00216-012-6466-9.

2012

Sophie Ayciriex, Anne Regazzetti, Mathieu Gaudin, Elise Prost, Delphine Dargère, et al. Development of a novel method for quantification of sterols and oxysterols by UPLC-ESI-HRMS: application to a neuroinflammation rat model. Anal Bioanal Chem. 2012 Dec;404(10):3049-59. doi: 10.1007/s00216-012-6396-6.

Sophie Ayciriex, Marina Le Guédard, Nadine Camougrand, Gisèle Velours, Mario Schoene, et al. YPR139c/LOA1 encodes a novel lysophosphatidic acid acyltransferase associated with lipid droplets and involved in TAG homeostasis. Mol Biol Cell. 2012 Jan;23(2):233-46. doi: 10.1091/mbc.E11-07-0650.

2011

Sophie Ayciriex, David Touboul, Alain Brunelle, Olivier Laprévote. Time-of-flight secondary ion mass spectrometer: a novel tool for lipid imaging. Clinical Lipidology 2011, 6 (4), 437-445

2009

Marina Le Guédard, Jean-Jacques Bessoule, Valérie Boyer, Sophie Ayciriex, Gisèle Velours, et al. PSI1 is responsible for the stearic acid enrichment that is characteristic of phosphatidylinositol in yeast. FEBS J. 2009 Nov;276(21):6412-24. doi: 10.1111/j.1742-4658.2009.07355.x.

Publications

Publications HAL de sophie,ayciriex

2017

Journal articles

ref_biblio
Blandine Rougemont, Sebastien Gallo, Sophie Ayciriex, Romain Carrière, Hubert Hondermarck, et al.. Scout-MRM: Multiplexed Targeted Mass Spectrometry-Based Assay without Retention Time Scheduling Exemplified by Dickeya dadantii Proteomic Analysis during Plant Infection. Analytical Chemistry, American Chemical Society, 2017, 89 (3), pp.1421-1426 <10.1021/acs.analchem.6b03201>. <hal-01518912>
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