Jerome LEMOINE

Professor
work phone: 0437423548

Research

Methodological and instrumental developments in mass spectrometry and hyphenated techniques for the relative or absolute multiplexed quantification of proteins in biological matrices : from proteomic discovery to clinical applications

improved detection specificity by coupling laser photodissociation to mass spectrometry or MRM3

increased multiplexing capability

Biography

Jérôme Lemoine (M) is Professor at Lyon 1 University, head of the bioMolecular Mass Spectrometry team in the Institut des Sciences Analytiques. Jérôme Lemoine was awarded a PhD from the University of Lille in 1993 for his work on high-energy fragmentation pathways of glycans by tandem mass spectrometry.
He was appointed an Associate Professor at Lille University in 1993, while following an internship of few months in the lab of Keith Jennings (Warwick University), then appointed a Professor of Biochemistry in 2001.
He moved to Lyon in 2004 with the objective of developing a multidisciplinary team in the field of biological mass spectrometry. Since then, his research topics have been focused on interdisciplinary mass spectrometry developments to improve the structural and quantitative analysis of biomolecules in the context of human diseases such as Alzheimer, cancer or high blood pressure.
He has been involved in the supervision or co-supervision of 15 PhD sudents.
Jérôme Lemoine has published 98 papers (H factor 30), filled 3 patents, and is co-founder of the start-up company AnaQuant.

Keywords

targeted mass spectrometry, laser photodissociation, ion mobility, protein/peptide biomarkers, clinical studies

Publications

Publications HAL de jerome,lemoine

2015

Journal articles

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Marion Girod, Zeljka Sanader, Marin Vojkovic, Rodolphe Antoine, Luke Macaleese, et al.. UV Photodissociation of proline-containing peptide ions: insights from molecular dynamics. Journal of The American Society for Mass Spectrometry, Springer Verlag (Germany), 2015, 26 (3), pp.432-443. <10.1007/s13361-014-1038-1>. <hal-01187318>
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Kevin Jeanne Dit Fouque, Hélène Lavanant, Séverine Zirah, Jérôme Lemoine, Sylvie Rebuffat, et al.. Gas-phase conformations of capistruin – comparison of lasso, branched-cyclic and linear topologies. Rapid Communications in Mass Spectrometry, Wiley, 2015, 29 (15), pp.1411-1419. <10.1002/rcm.7231>. <hal-01186671>
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Yannick Charretier, Olivier Dauwalder, Christine Franceschi, Elodie Degout-Charmette, Gilles Zambardi, et al.. Rapid bacterial identification, resistance, virulence and type profiling using selected reaction monitoring mass spectrometry. Scientific Reports, Nature Publishing Group, 2015, 5, pp.Article Number: 13944. <10.1038/srep13944>. <hal-01229864>
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Jordane Biarc, Romain Simon, Catherine Fonbonne, Jean-François Léonard, Jean-Charles Gauthier, et al.. Absolute quantification of podocalyxin, a potential biomarker of glomerular injury in human urine, by liquid chromatography-mass spectrometry. Journal of Chromatography A, Elsevier, 2015, 1397, pp.81-85. <10.1016/j.chroma.2015.04.003>. <hal-01164871>
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Blandine Rougemont, Romain Simon, Romain Carrière, Jordane Biarc, Catherine Fonbonne, et al.. Absolute quantification of dengue virus serotype 4 chimera vaccine candidate in Vero cell culture by targeted mass spectrometry. Proteomics, Wiley-VCH Verlag, 2015, 15 (19), pp.3320-3330. <10.1002/pmic.201500101>. <hal-01229336>
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2014

Journal articles

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David Ropartz, Jérôme Lemoine, Alexandre Giuliani, Yann Bittebiere, Quentin Enjalbert, et al.. Deciphering the structure of isomeric oligosaccharides in a complex mixture by tandem mass spectrometry: photon activation with vacuum ultra-violet brings unique information and enables definitive structure assignment.. Analytica Chimica Acta, Elsevier Masson, 2014, 807, pp.84-95. <10.1016/j.aca.2013.11.018>. <hal-00954660>
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Jéremy Jeudy, Arnaud Salvador, Romain Simon, Aurore Jaffuel, Catherine Fonbonne, et al.. Overcoming biofluid protein complexity during targeted mass spectrometry detection and quantification of protein biomarkers by MRM cubed (MRM3). Analytical and Bioanalytical Chemistry, Springer Verlag, 2014, 406 (4), pp.1193-1200. <10.1007/s00216-013-7266-6>. <hal-00954963>
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Romain Simon, Jérôme Lemoine, Catherine Fonbonne, Aurore Jaffuel, Jean-François Léonard, et al.. Absolute quantification of podocin, a potential biomarker of glomerular injury in human urine, by liquid chromatography-multiple reaction monitoring cubed mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis, Elsevier, 2014, 94, pp.84-91. <10.1016/j.jpba.2014.01.019>. <hal-00954969>
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Jordane Biarc, Philippe Gonzalo, Ivan Mikaelian, Laurent Fattet, Mathieu Deygas, et al.. Combination of a discovery LC-MS/MS analysis and a label-free quantification for the characterization of an epithelial-mesenchymal transition signature. Journal of Proteomics, Elsevier, 2014, 110, pp.183-194. <10.1016/j.jprot.2014.05.026>. <hal-01230272>
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Marion Girod, Marjan Krstić, Rodolphe Antoine, Luke Macaleese, Jérôme Lemoine, et al.. Formation and characterisation of the silver hydride nanocluster cation [Ag3H2((Ph2P)(2)CH2)](+) and its release of hydrogen. Chemistry – A European Journal, Wiley-VCH Verlag, 2014, 20 (50), pp.16626-16633. <10.1002/chem.201404110>. <hal-01230129>
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Marion Girod, Quentin Enjalbert, Claire Brunet, Rodolphe Antoine, Jérôme Lemoine, et al.. Structural basis of protein oxidation resistance: a lysozyme study.. PLoS ONE, Public Library of Science, 2014, 9 (7), pp.e101642. <10.1371/journal.pone.0101642>. <hal-01071810>
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Romain Simon, Sébastien Passeron, Jérôme Lemoine, Arnaud Salvador. Hydrophilic interaction liquid chromatography as second dimension in multidimensional chromatography with an anionic trapping strategy: application to prostate-specific antigen quantification.. Journal of Chromatography A, Elsevier, 2014, 1354, pp.75-84. <10.1016/j.chroma.2014.05.063>. <hal-01071821>
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Marion Girod, Jordane Biarc, Quentin Enjalbert, Arnaud Salvador, Rodolphe Antoine, et al.. Implementing visible 473 nm photodissociation in a Q-Exactive mass spectrometer: towards specific detection of cysteine-containing peptides. Analyst, Royal Society of Chemistry, 2014, 139 (21), pp.5523-5530. <10.1039/c4an00956h>. <hal-01234356>
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2013

Journal articles

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Marie Pérot-Taillandier, Séverine Zirah, Quentin Enjalbert, Rodolphe Antoine, Jérôme Lemoine, et al.. Prompt and slow electron-detachment-dissociation/electron-photodetachment-dissociation of a 21-mer Peptide.. Chemistry – A European Journal, Wiley-VCH Verlag, 2013, 19 (1), pp.350-7. <10.1002/chem.201202578>. <hal-00770793>
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Marion Girod, Rodolphe Antoine, Jérôme Lemoine, Philippe Dugourd, Laurence Charles. Structural characterization of a poly(methacrylicacid)/poly(methylmethacrylate) copolymer by activated electron photo-detachment dissociation. International Journal of Mass Spectrometry, Elsevier, 2013, 333, pp.27-33. <10.1016/j.ijms.2012.08.018>. <hal-00875704>
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Floriane Pailleux, Jérôme Lemoine, Francis Beaudry. Investigation of the metabolic biotransformation of substance P in liver microsomes by liquid chromatography quadrupole ion trap mass spectrometry. Biomedical Chromatography, Wiley, 2013, 27 (1), pp.39-47. <10.1002/bmc.274>. <hal-00875709>
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Le Thi Nga, Jean Christophe Poully, Frédéric Lecomte, Nicolas Nieuwjaer, Bruno Manil, et al.. Gas-phase structure of amyloid-β (12 – 28) peptide investigated by infrared spectroscopy, electron capture dissociation and ion mobility mass spectrometry.. Journal of The American Society for Mass Spectrometry, Springer Verlag (Germany), 2013, 24 (12), pp.1937-1949. <10.1007/s13361-013-0722-x>. <hal-00924163>
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Rodolphe Antoine, Jérôme Lemoine, Philippe Dugourd. Electron photodetachment dissociation for structural characterization of synthetic and bio-polymer anions. Mass Spectrometry Reviews, Wiley, 2013, <10.1002/mas.21402>. <hal-00954983>
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Floriane Pailleux, Pascal Vachon, Jérôme Lemoine, Francis Beaudry. Targeted liquid chromatography quadrupole ion trap mass spectrometry analysis of tachykinin related peptides reveals significant expression differences in a rat model of neuropathic pain.. Neuropeptides, Elsevier, 2013, 47 (4), pp.261-271. <10.1016/j.npep.2013.02.001>. <hal-00851139>
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Aurore Jaffuel, Jérôme Lemoine, Catherine Aubert, Romain Simon, Jean-Francois Léonard, et al.. Optimization of liquid chromatography-multiple reaction monitoring cubed mass spectrometry assay for protein quantification: Application to aquaporin-2 water channel in human urine. Journal of Chromatography A, Elsevier, 2013, 1301, pp.122-130. <10.1016/j.chroma.2013.05.068>. <hal-00860529>
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Arnaud Vernier, Fabien Chirot, Rodolphe Antoine, Arnaud Salvador, Jean-Philippe Charrier, et al.. Alternative representation for the stability diagram of quadrupole ion traps upon additional quadrupolar excitation. European Journal of Mass Spectrometry, IM Publications, 2013, 19 (3), pp.141-149. <10.1255/ejms.122>. <hal-00873924>
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Quentin Enjalbert, Marion Girod, Romain Simon, Jéremy Jeudy, Fabien Chirot, et al.. Improved detection specificity for plasma proteins by targeting cysteine-containing peptides with photo-SRM. Analytical and Bioanalytical Chemistry, Springer Verlag, 2013, 405 (7), pp.2321-2331. <10.1007/s00216-012-6603-5>. <hal-00875276>
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Quentin Enjalbert, Claire Brunet, Arnaud Vernier, Abdul-Rahman Allouche, Rodolphe Antoine, et al.. Vacuum ultraviolet action spectroscopy of polysaccharides.. Journal of The American Society for Mass Spectrometry, Springer Verlag (Germany), 2013, 24 (8), pp.1271-1279. <10.1007/s13361-013-0657-2>. <hal-00864641>
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2012

Journal articles

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Claire Brunet, Rodolphe Antoine, Jérôme Lemoine, Philippe Dugourd. Soret band of the gas-phase ferri-cytochrome c. Journal of Physical Chemistry Letters, American Chemical Society (ACS), 2012, 3 (6), pp.698-702. <10.1021/jz300070r>. <hal-00875736>
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Jérôme Lemoine, Tanguy Fortin, Arnaud Salvador, Aurore Jaffuel, Jean-Philippe Charrier, et al.. The current status of clinical proteomics and the use of MRM and MRM3 for biomarker validation. Expert Review of Molecular Diagnostics, Expert Reviews (formerly Future Drugs), 2012, 12 (4), pp.333-342. <10.1586/ERM.12.32>. <hal-00875727>
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Fabien Chirot, Jérôme Lemoine, Luke Mac Aleese, Philippe Dugourd. Spectrométrie par mobilité ionique et agrégation des protéines. Spectra Analyse, 2012, 285, pp.64. <hal-00963599>
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Fabien Chirot, Florent Calvo, Florian Albrieux, Jérôme Lemoine, Yury O. Tsybin, et al.. Statistical analysis of ion mobility spectrometry. I. unbiased and guided replica-exchange molecular dynamics. Journal of The American Society for Mass Spectrometry, Springer Verlag (Germany), 2012, 23 (2), pp.386-396. <10.1007/s13361-011-0281-y>. <hal-00873906>
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Florent Calvo, Fabien Chirot, Florian Albrieux, Jérôme Lemoine, Yury O. Tsybin, et al.. The current status of clinical proteomics and the use of MRM and MRM(3) for biomarker validation.. Expert Review of Molecular Diagnostics, Expert Reviews (formerly Future Drugs), 2012, 12 (4), pp.333-42. <10.1586/erm.12.32>. <hal-00873914>
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Bruno Bellina, Isabelle Compagnon, Luke Macaleese, Fabien Chirot, Jérôme Lemoine, et al.. Binding motifs of silver in prion octarepeat model peptides: a joint ion mobility, IR and UV spectroscopies, and theoretical approach. Physical Chemistry Chemical Physics, Royal Society of Chemistry, 2012, 14 (32), pp.11433-11440. <10.1039/c2cp40924k>. <hal-00873876>
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Romain Simon, Quentin Enjalbert, Jordane Biarc, Jérôme Lemoine, Arnaud Salvador. Evaluation of hydrophilic interaction chromatography (HILIC) versus C-18 reversed-phase chromatography for targeted quantification of peptides by mass spectrometry. Journal of Chromatography A, Elsevier, 2012, 1264, pp.31-39. <10.1016/j.chroma.2012.09.059>. <hal-00875257>
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Quentin Enjalbert, Amandine Racaud, Jérôme Lemoine, Sébastien Redon, Mehmet Menaf Ayhan, et al.. Optical properties of a visible push-pull chromophore covalently bound to carbohydrates: solution and gas-phase spectroscopy combined to theoretical Investigations. Journal of Physical Chemistry B, American Chemical Society, 2012, 116 (2), pp.841-851. <10.1021/jp2099015>. <hal-00875784>
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Floriane Pailleux, Jérôme Lemoine, Francis Beaudry. Quantitative mass Spectrometry analysis reveals that deletion of the TRPV1 receptor in mice alters substance P and Neurokinin A expression in the central nervous system. Neurochemical Research, Springer Verlag, 2012, 37 (12), pp.2678-2685. <10.1007/s11064-012-0856-4>. <hal-00875718>
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Marion Girod, Claire Brunet, Rodolphe Antoine, Jérôme Lemoine, Philippe Dugourd, et al.. Efficient structural characterization of poly(methacrylic acid) by activated-electron photodetachment dissociation.. Journal of The American Society for Mass Spectrometry, Springer Verlag (Germany), 2012, 23 (1), pp.7-11. <10.1007/s13361-011-0279-5>. <hal-00875927>
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Romain Simon, Marion Girod, Catherine Fonbonne, Arnaud Salvador, Yohann Clément, et al.. Total ApoE and ApoE4 isoform assays in an alzheimer’s disease case-control study by targeted mass spectrometry (n=669): a pilot assay for methionine-containing proteotypic peptides. Molecular and Cellular Proteomics, American Society for Biochemistry and Molecular Biology, 2012, 11 (11), pp.1389-1403. <10.1074/mcp.M112.018861>. <hal-00875286>
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2011

Journal articles

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Quentin Enjalbert, Romain Simon, Arnaud Salvador, Rodolphe Antoine, Sébastien Redon, et al.. Photo-SRM: laser-induced dissociation improves detection selectivity of Selected Reaction Monitoring mode.. Rapid Communications in Mass Spectrometry, Wiley, 2011, 25 (22), pp.3375-0081. <10.1002/rcm.5232>. <hal-00875933>
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Francis Canon, Renaud Ballivian, Fabien Chirot, Rodolphe Antoine, Pascale Sarni-Manchado, et al.. Folding of a salivary intrinsically disordered protein upon binding to tannins. Journal of the American Chemical Society, American Chemical Society, 2011, 133 (20), pp.7847-7852. <10.1021/ja200534f>. <hal-00875405>
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Julien De Winter, Vincent Lemaur, Renaud Ballivian, Fabien Chirot, Olivier Coulembier, et al.. Size dependence of the folding of multiply charged sodium cationized polylactides revealed by ion mobility mass spectrometry and molecular modelling. Chemistry – A European Journal, Wiley-VCH Verlag, 2011, 17 (35), pp.9738-9745. <10.1002/chem.201100383>. <hal-00875409>
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Florian Albrieux, Hisham Ben Hamidane, Florent Calvo, Fabien Chirot, Yury O. Tsybin, et al.. Structural preferences of gas-phase M2TMP monomers upon sequence variations. Journal of Physical Chemistry A, American Chemical Society, 2011, 115 (18), pp.4711-4718. <10.1021/jp110732>. <hal-00875402>
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Marion Girod, Rodolphe Antoine, Jérôme Lemoine, Philippe Dugourd, Laurence Charles. End-group characterization of poly(styrene sulfonate sodium salt) by activated electron photo-detachment dissociation.. Rapid Communications in Mass Spectrometry, Wiley, 2011, 25 (21), pp.3259-3266. <10.1002/rcm.5228>. <hal-00875937>
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