Fabien CHIROT

Doctor
phone: 0437423678

Research

Gas phase approaches to the structural properties of biomolecules and biomolecular complexes. Instrumental developments for ion mobility spectrometry, mass spectrometry, and their combination with laser spectroscopy. Methodological developments for the structural interpretation of ion mobility measurements.

Biography

Fabien Chirot is currently Maître de Conférences (assistant professor) at Université Lyon 1, in the Biological Analysis by Mass Spectrometry group. He received his PhD in Physics at the University Toulouse III in 2007, after experimental works on the nucleation processes, and on the thermodynamics of phase transitions in small atomic clusters. From 2007 to 2008 he worked as a post-doctoral fellow in the group of Pr. von Issendorff at the University of Freiburg (Germany), where he investigated the solvation of an electron in small water clusters using photoelectron spectroscopy. He was recruited at Université Lyon 1 in 2008. Since then he has been in charge of the ion mobility spectrometry activities at ISA, in close collaboration with the group of P. Dugourd (ILM).

Keywords

Ion Mobility Spectrometry, Mass Spectrometry, Molecular Dynamics, Action Spectroscopy, Förster Resonance Energy Transfer, Structure and Dynamics of Biomolecules, Biomolecular Complexes

Publications

Publications HAL de fabien,chirot

2016

Journal articles

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Alexander Kulesza, Steven Daly, Chang Min Choi, Anne-Laure Simon, Fabien Chirot, et al.. The structure of chromophore-grafted amyloid-beta(12-28) dimers in the gas-phase: FRET-experiment guided modelling. Physical Chemistry Chemical Physics, Royal Society of Chemistry, 2016, 18 (13), pp.9061-9069. <10.1039/c6cp00263c>. <hal-01356201>
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Chang Min Choi, Anne-Laure Simon, Fabien Chirot, Alexander Kulesza, Geoffrey Knight, et al.. Charge, color, and conformation: spectroscopy on isomer-selected peptide ions. Journal of Physical Chemistry B, American Chemical Society, 2016, 120 (4), pp.709-714. <10.1021/acs.jpcb.5b11919>. <hal-01356301>
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Valéria Lepère, Katia Le Barbu-Debus, Carine Clavaguera, Deborah Scuderi, Giovanni Piani, et al.. Chirality-dependent structuration of protonated or sodiated polyphenylalanines: IRMPD and ion mobility studies. Physical Chemistry Chemical Physics, Royal Society of Chemistry, 2016, 18 (3), pp.1807-18017. <10.1039/c5cp06768e>. <hal-01356198>
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Alexander Kulesza, Steven Daly, Chang Min Choi, Anne-Laure Simon, Fabien Chirot, et al.. The structure of chromophore-grafted Amyloid-β 12-28 dimers in the gas-phase: FRET-experiments guided modelling. PCCP : Physical chemistry chemical physics, Royal Society of Chemistry, 2016, <10.1039/c6cp00263c>. <hal-01344513>
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https://hal.archives-ouvertes.fr/hal-01344513/file/The%20structure%20of%20chromophore-grafted%20Amyloid%20B%20dimers%20the%20gas%20phase.pdf https://hal.archives-ouvertes.fr/hal-01344513/file/SuplementaryInformation_Proofstage_250216.pdf BibTex
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Chang Min Choi, Anne-Laure Simon, Fabien Chirot, Alexander Kulesza, Geoffrey Knight, et al.. Charge, Color, and Conformation: Spectroscopy on Isomer-Selected Peptide Ions. Journal of Physical Chemistry B, American Chemical Society, 2016, 120 (4), pp.709-714. <10.1021/acs.jpcb.5b11919>. <hal-01345016>
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https://hal.archives-ouvertes.fr/hal-01345016/file/EyPeptide.pdf BibTex

2015

Journal articles

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Anne-Laure Simon, Fabien Chirot, Chang Min Choi, Christian Clavier, Marc Barbaire, et al.. Tandem ion mobility spectrometry coupled to laser excitation. Review of Scientific Instruments, American Institute of Physics, 2015, 86 (9), pp.Article Number: 094101. <10.1063/1.4930604>. <hal-01229281>
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Kevin Jeanne Dit Fouque, Hélène Lavanant, Séverine Zirah, Jérôme Lemoine, Sylvie Rebuffat, et al.. Gas-phase conformations of capistruin – comparison of lasso, branched-cyclic and linear topologies. Rapid Communications in Mass Spectrometry, Wiley, 2015, 29 (15), pp.1411-1419. <10.1002/rcm.7231>. <hal-01186671>
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Anne-Laure Simon, Fabien Chirot, Chang Min Choi, Christian Clavier, Marc Barbaire, et al.. Tandem ion mobility spectrometry coupled to laser excitation. Review of Scientific Instruments, American Institute of Physics, 2015, <10.1063/1.4930604>. <hal-01344740>
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https://hal.archives-ouvertes.fr/hal-01344740/file/IMS%20Laser%20IMS.pdf BibTex
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Steven Daly, Alexander Kulesza, Frédéric Poussigue, Anne-Laure Simon, Chang Min Choi, et al.. Conformational changes in amyloid-beta (12-28) alloforms studied using action-FRET, IMS and molecular dynamics simulations. Chemical Science , The Royal Society of Chemistry, 2015, 6 (8), pp.5040-5047. <10.1039/c5sc01463h>. <hal-01187453>
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2014

Journal articles

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Steven Daly, Frédéric Poussigue, Anne-Laure Simon, Luke Macaleese, Franck Bertorelle, et al.. Action-FRET: Probing the Molecular Conformation of Mass-Selected Gas-Phase Peptides with Forster Resonance Energy Transfer Detected by Acceptor-Specific Fragmentation. Analytical Chemistry, American Chemical Society, 2014, 86 (17), pp.8798-8804. <10.1021/ac502027y>. <hal-01070732>
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Jean-Yves Salpin, Luke Macaleese, Fabien Chirot, Philippe Dugourd. Structure of the Pb²⁺-deprotonated dGMP complex in the gas phase: a combined MS-MS/IRMPD spectroscopy/ion mobility study.. Physical Chemistry Chemical Physics, Royal Society of Chemistry, 2014, 16 (27), pp.14127-14138. <10.1039/c4cp00163j>. <hal-01071755>
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Jean-Yves Salpin, Luke Macaleese, Fabien Chirot, Philippe Dugourd. Structure of Pb 2+ /deprotonated dGMP complexes in the gas phase: a combined MS-MS/IRMPD spectroscopy/Ion Mobility study. Physical Chemistry Chemical Physics, Royal Society of Chemistry, 2014, pp.14127-14138. <10.1039/C4CP00163J>. <hal-01345026>
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https://hal.archives-ouvertes.fr/hal-01345026/file/Pb2%2BdGMP.pdf https://hal.archives-ouvertes.fr/hal-01345026/file/Pb2%2BdGMP_Supporting%20information.pdf BibTex
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Matthias Vonderach, Marc-Oliver Winghart, Luke Macaleese, Fabien Chirot, Rodolphe Antoine, et al.. Conformer-selective photoelectron spectroscopy of alpha-lactalbumin derived multianions in the gas phase. Physical Chemistry Chemical Physics, Royal Society of Chemistry, 2014, 16 (7), pp.3007-3013. <10.1039/c3cp54596b>. <hal-00954595>
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Steven Daly, Frédéric Poussigue, Anne-Laure Simon, Luke Macaleese, Franck Bertorelle, et al.. Action-FRET: Probing the molecular conformation of mass-selected gas phase peptides with Förster Resonance Energy Transfer detected by acceptor specific fragmentation. Analytical Chemistry, American Chemical Society, 2014, <10.1021/ac502027y>. <hal-01344561>
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https://hal.archives-ouvertes.fr/hal-01344561/file/ActionFRET_analchem.pdf BibTex

2013

Journal articles

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Le Thi Nga, Jean Christophe Poully, Frédéric Lecomte, Nicolas Nieuwjaer, Bruno Manil, et al.. Gas-phase structure of amyloid-β (12 – 28) peptide investigated by infrared spectroscopy, electron capture dissociation and ion mobility mass spectrometry.. Journal of The American Society for Mass Spectrometry, Springer Verlag (Germany), 2013, 24 (12), pp.1937-1949. <10.1007/s13361-013-0722-x>. <hal-00924163>
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Vincent Lemaur, Glenn Carroy, Frédéric Poussigue, Fabien Chirot, Julien De Winter, et al.. Homotropic allosterism: in-depth structural analysis of the gas-phase noncovalent complexes associating a double-cavity cucurbit[n]uril-type host and size-selected protonated amino compounds. ChemPlusChem, 2013, 78 (9), pp.959-969. <10.1002/cplu.201300208>. <hal-00873938>
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Quentin Enjalbert, Marion Girod, Romain Simon, Jéremy Jeudy, Fabien Chirot, et al.. Improved detection specificity for plasma proteins by targeting cysteine-containing peptides with photo-SRM. Analytical and Bioanalytical Chemistry, Springer Verlag, 2013, 405 (7), pp.2321-2331. <10.1007/s00216-012-6603-5>. <hal-00875276>
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Arnaud Vernier, Fabien Chirot, Rodolphe Antoine, Arnaud Salvador, Jean-Philippe Charrier, et al.. Alternative representation for the stability diagram of quadrupole ion traps upon additional quadrupolar excitation. European Journal of Mass Spectrometry, IM Publications, 2013, 19 (3), pp.141-149. <10.1255/ejms.122>. <hal-00873924>
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2012

Journal articles

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Florent Calvo, Fabien Chirot, Florian Albrieux, Jérôme Lemoine, Yury O. Tsybin, et al.. The current status of clinical proteomics and the use of MRM and MRM(3) for biomarker validation.. Expert Review of Molecular Diagnostics, Expert Reviews (formerly Future Drugs), 2012, 12 (4), pp.333-42. <10.1586/erm.12.32>. <hal-00873914>
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Fabien Chirot, Florent Calvo, Florian Albrieux, Jérôme Lemoine, Yury O. Tsybin, et al.. Statistical analysis of ion mobility spectrometry. I. unbiased and guided replica-exchange molecular dynamics. Journal of The American Society for Mass Spectrometry, Springer Verlag (Germany), 2012, 23 (2), pp.386-396. <10.1007/s13361-011-0281-y>. <hal-00873906>
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Fabien Chirot, Jérôme Lemoine, Luke Mac Aleese, Philippe Dugourd. Spectrométrie par mobilité ionique et agrégation des protéines. Spectra Analyse, Paris : PCI, 2012, 285, pp.64. <hal-00963599>
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Bruno Bellina, Isabelle Compagnon, Luke Macaleese, Fabien Chirot, Jérôme Lemoine, et al.. Binding motifs of silver in prion octarepeat model peptides: a joint ion mobility, IR and UV spectroscopies, and theoretical approach. Physical Chemistry Chemical Physics, Royal Society of Chemistry, 2012, 14 (32), pp.11433-11440. <10.1039/c2cp40924k>. <hal-00873876>
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2011

Journal articles

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Julien De Winter, Vincent Lemaur, Renaud Ballivian, Fabien Chirot, Olivier Coulembier, et al.. Size dependence of the folding of multiply charged sodium cationized polylactides revealed by ion mobility mass spectrometry and molecular modelling. Chemistry – A European Journal, Wiley-VCH Verlag, 2011, 17 (35), pp.9738-9745. <10.1002/chem.201100383>. <hal-00875409>
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Florian Albrieux, Hisham Ben Hamidane, Florent Calvo, Fabien Chirot, Yury O. Tsybin, et al.. Structural preferences of gas-phase M2TMP monomers upon sequence variations. Journal of Physical Chemistry A, American Chemical Society, 2011, 115 (18), pp.4711-4718. <10.1021/jp110732>. <hal-00875402>
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Francis Canon, Renaud Ballivian, Fabien Chirot, Rodolphe Antoine, Pascale Sarni-Manchado, et al.. Folding of a salivary intrinsically disordered protein upon binding to tannins. Journal of the American Chemical Society, American Chemical Society, 2011, 133 (20), pp.7847-7852. <10.1021/ja200534f>. <hal-00875405>
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Sébastien Zamith, Pierre Labastie, Fabien Chirot, Jean-Marc L’Hermite. Erratum: “Two step melting of Na41+” [J. Chem. Phys. 133, 154501 (2010)]. Journal of Chemical Physics, American Institute of Physics, 2011, 134 (12), pp.129902. <10.1063/1.3562377>. <hal-00580742>
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