silica-capillary-monolith

Based on both thermodynamic and kinetic approaches of transfer processes in separation tools, the group develops separation methodologies to tackle characterization of complex samples within the fields of environment, health, nuclear and industrial analysis. Research is driven by the reduction in size of separation devices and the development of innovative stationary phases (monolith) and coatings dedicated to miniaturized sample preparation, gas chromatography, nano-liquid chromatography, electrochromatography and capillary electrophoresis as well as to their in-line coupling with highly sensitive and specific ICP-MS and nano-LC-MS detectors.

Miniaturization of separation systems
Miniaturized analytical systems are required when experimental constraints are extreme, such as low sample volume (in-vivo life science), hazardous sample (nuclear technology) or highly complex samples (health, environment or bioprocess). The group identifies relevant descriptors for the design of materials and separation microsystems and optimizes complex separation systems using monolithic stationary phases based on silica that can be photochemically in-situ synthesized and withstand extreme analytical conditions.

Innovative stationary phases and coatings
Expert in the field of synthesis of inorganic monolithic stationary phases, especially synthesis inside capillary tubing, the group improves stationary phases dedicated to nano-liquid chromatography as well as to miniaturized sample preparation or their in-line coupling. It also develops original, rapid, localizable and versatile photochemical grafting processes (i.e. photopolymerization, photo-click grafting) to tune their surface properties, thus allowing the preparation of columns and sampling devices with multimodal interactions for multidimensional analysis

Recent highlights

  • Monolithic silica columns with high length for ultra high resolution liquid chromatography (Column 1.6 m, 260 000 plates)
  • Quantitative immobilization of aptamers on monolith by photoclick chemistry
  • Method for manufacturing a multi-modal device comprising a porous monolith contained in a micro-fluidic circuit by photo-grafting. (Patent 2013/11/29, FR1361833)

Contact: Pr. Jérôme RANDON, Group leader – jerome.randon@isa-lyon.fr