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The group develops analytical methods, based on both targeted and untargeted approaches by mass spectrometry, to study the exposure of environmental ecosystems and human beings to organic micropollutants and their impact on Health.

We develop innovative methods involving improvements at each step of the analytical protocol: sampling, sample preparation, chromatographic separation, detection by mass spectrometry and data analysis (passive sampler, dSPE, SPE-LC-MS/MS online, µ-QuEChERS, SALLE, nano-LC-MS/MS, LC- or GC-HRMS…)

  • Concerning targeted analysis, we quantify trace and ultra-trace of priority or emerging contaminants, i.e. drugs, hormones, pesticides, endocrine disruptors, and of their transformation products and metabolites in environmental matrices (water, soil, sediment, sludge, sentinel species, …) or biological fluids (blood, urine, serum, tissues…). Our developments aim at lowering the limits of quantification, enhancing selectivity or reducing the sample size.
  • For the detection and identification of biomarkers or marker of exposures to these contaminants, we develop analytical methodologies based on comprehensive and untargeted analysis or metabolomic approaches, by LC- or GC-HRMS.

The combination of both targeted and non-targeted analytical strategies allows improvement of the understanding of the Environment-Health interactions, concept known as Exposome, for environmental and health issues.

 

Recent highlights

  • Contribution to a national study on the occurrence of biocides and surfactants in aquatic environments – EMNAT (AFB)
  • Assessment of the bioaccumulation and metabolisation of emerging pharmaceutical compounds and pesticides in sentinel species – DOREMIPHARM (ANSM) and MAIIA (Rhone-Alpes Region)
  • Development of a metabolomic approach to study the impact of pharmaceutical compounds on small aquatic invertebrates, at individual level – ANABIOTE (CNRS) and collaboration with VEOLIA
  • Assessment of the impact of plastic additives on the development of breath cancer – PLASTOC (INSERM)
  • Development of new passive samplers to improve the analysis of groundwaters – FROG (ANR)

Contact: Dr Emmanuelle VULLIET, Group leader – emmanuelle.vulliet@isa-lyon.fr